Improvement of Phenolic Compounds and Antihyperlipidemic Activity of <i>Hibiscus sabdariffa</i> L. Calyxes Powder Using CDS Processing

Linda Stella Mbassi; Elie Baudelaire Djantou; Richard Marcel Nguimbou; Amadou Dicko; Nicolas Yanou Njintang

doi:doi:10.11648/j.ajls.20231102.13

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Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing

Linda Stella Mbassi, Elie Baudelaire Djantou, Richard Marcel Nguimbou, Amadou Dicko, Nicolas Yanou Njintang

Published in American Journal of Life Sciences (Volume 11, Issue 2)

Received: 10 March 2022 Accepted: 11 August 2022 Published: 9 June 2023

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Abstract

The aim of this study was to evaluate the effect of controlled differential sieving processing (CDSp) on the content of phenolic compounds and antihyperlipidemic activity of H. sabdariffa calyxes powder. For this, four granulometric classes (<180 μm, 180-212 μm, 212-315 μm, ≥315 μm) were analyzed. Unsieved powder and lyophilized ethanolic extract were used for comparison. First, we identified and quantified the phenolic compounds present in the samples by chromatography in liquid phase coupled to a UV detector and mass spectrometry (LC-MS). Second, we evaluated the antihyperlipidemic activity of the different fractions in adult male rats at a dose of 250 mg/kg and compared them with those of unsieved powder and the ethanolic extract. LC-MS analysis identified seven compounds (quercetin, rutin, catechin, ferrulic acid, p-coumaric acid, protocatechic acid and cafeic acid) in the different sample of H. sabdariffa calyxes powder. The granulometric class 212-315 μm showed the best concentration, higher than those of the other granulometric class and crude powder. This fraction also showed content of phenolic compounds higher than those of ethanolic extract except for p-coumaric acid, and protocatechic acid which were concentrated equally in both samples. Antihyperlipidemic activity were significantly influenced (P <0.05) by particle sizes. The best activity was obtained in the particle size classes of 212-315 µm and <180 µm which were significantly (P <0.05) higher than those of the other fractions and of the unsieved powder to improve the lipid parameters (total cholesterol, LDL-C, HDL-C and triglycerides), transaminases (ALAT and ASAT) and creatine. However, compared to the ethanolic extract, only the granulometric class of 212-315 µm showed a significantly higher activity. CDSp can be an alternative to ethanol extraction and may be an option to improve the content of phenolic compounds and antihyperlipidemic activity of plant powders.

Published in	American Journal of Life Sciences (Volume 11, Issue 2)
DOI	10.11648/j.ajls.20231102.13
Page(s)	24-35
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Antihyperlipidemic Activity, Hibiscus sabdariffa Calyxes, Powder Fraction, Lyophilized Ethanolic Extract, Phenolic Compounds

References

[1]	Onat, A. (2001). Risk factors and cardiovascular disease in Turkey. Atherosclerosis, 156 (1), 1-10. https://doi.org/10.1016/S0021-9150(01)00500-7
[2]	Thirumalai, T., Tamilselvan, N., & David, E. (2014). Hypolipidemic activity of Piper betel in high fat diet induced hyperlipidemic rat. Journal of Acute Disease, 3 (2), 131-135. https://doi.org/10.1016/S2221-6189(14)60029-9
[3]	Girija, K., & Lakshman, K. (2011). Anti-hyperlipidemic activity of methanol extracts of three plants of Amaranthus in triton-WR 1339 induced hyperlipidemic rats. Asian Pacific Journal of Tropical Biomedicine, 1 (1), S62-S65. https://doi.org/10.1016/S2221-1691(11)60125-1
[4]	Henley, E., Chang, L., & Hollander, S. (2002). Treatment of hyperlipidemia. (Applied Evidence). Journal of family practice, 51 (4), 370-377.
[5]	Boudjeltia, K. Z., Moguilevsky, N., Legssyer, I., Babar, S., Guillaume, M., Delree, P., & Remacle, C. (2004). Oxidation of low density lipoproteins by myeloperoxidase at the surface of endothelial cells: an additional mechanism to subendothelium oxidation. Biochemical and biophysical research communications, 325 (2), 434-438. https://doi.org/10.1016/j.bbrc.2004.10.049
[6]	Abdelgadir, N. A., Salih, A. M., & Ayoub, S. M. (2019). Hypolipidemic effect of ethanolic extracts of Adansonia digitata fruit pulp on hyperlipidemic albino rats. The Journal of Phytopharmacology 2019; 8 (6): 279-281. 10.31254/phyto.2019.8601.
[7]	Ulla, A., Alam, M. A., Sikder, B., Sumi, F. A., Rahman, M. M., Habib, Z. F. & Reza, H. M. (2017). Supplementation of Syzygium cumini seed powder prevented obesity, glucose intolerance, hyperlipidemia and oxidative stress in high carbohydrate high fat diet induced obese rats. BMC complementary and alternative medicine, 17 (1), 1-13. https://doi.org/10.1186/s12906-017-1799-8
[8]	Sinela, A., Rawat, N., Mertz, C., Achir, N., Fulcrand, H., & Dornier, M. (2017). Anthocyanins degradation during storage of Hibiscus sabdariffa extract and evolution of its degradation products. Food Chemistry, 214, 234-241. https://doi.org/10.1016/j.foodchem.2016.07.071
[9]	Ismail, A., Ikram, E. H. K., & Nazri, H. S. M. (2008). Roselle (Hibiscus sabdariffa L.) seeds-nutritional composition, protein quality and health benefits. Food, 2 (1), 1-16.
[10]	Deli, M., Djantou, E. B., Ngatchic, M. J. T., Petit, J., Njintang, Y. N. and Scher, J. (2019a) Successive Grinding and Sieving as a New Tool to Fractionate Polyphenols and Antioxidants of Plants Powders: Application to Boscia senegalensis Seeds, Dichrostachys glomerata Fruits, and Hibiscus sabdariffa Calyx Powders. Food Science and Nutrition, 7, 1795-1806. https://doi.org/10.1002/fsn3.1022
[11]	Ameer, K., Shahbaz, H. M., and Kwon, J. H. (2017). Green extraction methods for polyphenols from plant matrices and their by products: a review. Comprehensive Reviews in Food Science and Food Safety, 16: 295–31. https://doi.org/10.1111/1541-4337.12253
[12]	Monroy, Y. M., Rodriguesb, R. A. F., Sartorattob, A., and Cabral, F. A. (2016). Optimization of the extraction of phenolic compounds from purplecorn cob (Zea mays L.) by sequential extraction using supercriticalcarbon dioxide, ethanol and water as solvents. Journal of Supercritical Fluids, 116: 10–19. 10.1016/j.supflu.2016.04.011.
[13]	Leonardo, L. P., Feitoza, G. S., and Costa, J. G. (2013). Development and validation of a HPLC method for the quantification of three flavonoids in a crude extract of Dimorphandra gardneriana. Revista Brasileira de Farmacognosia, 23 (1): 58-64.
[14]	Baudelaire, E. D. (2013). Comminution and controlled differential screening method for the dry extraction of natural active principles. Google patent WO2013057379A1.
[15]	Becker, L., Zaiter, A., Petit, J., Zimmer, D., Karam, M.-C., Baudelaire, E., Dicko, A. (2016). Improvement of antioxidant activity and polyphenol content of Hypericum perforatum and Achillea millefolium powders using successive grinding and sieving. Industrial Crops Products, 87, 116–123. https://doi.org/10.1016/j.indcrop.2016.04.036
[16]	Deli, M., Nguimbou, R. M., Njantou Baudelaire, E., Njintang Yanou, N., Scher, J., & Mbofung, C. M. (2020). Effect of controlled differential sieving processing on micronutrient contents and in vivo antioxidant activities of Hibiscus sabdariffa L. calyxes powder. Food science and biotechnology, 29, 1741–1753. https://doi.org/10.1002/fsn3.1022 9
[17]	Cujic´, N., Šavikin, K., Jankovic´, T., Pljevljakušic´, D., Zdunic´, G., and Ibric´ S. (2015). Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. Food Chemistry, 194: 135–142. https://doi.org/10.1016/j.foodchem.2015.08.008
[18]	Hamlat, N., Neggazi, S., Benazzo, Y., Kacimi, G., Chaid, S. and Aouichat-Bouguerra, S. (2008). Hyperlipidic diet and atherosclerosis process in Rattus norvegicus. Science and Technology, 27: 49-56.
[19]	Bourely, J. (1982). Observation on the dosage of cottonseed oil. Cotton and Tropical Fibers. 27: 183-196.
[20]	Naito, M., Kuzuya, F., Asai, K., and Yoshimine, N. (1984). Ineffectiveness of Ca²⁺ antagonists nicardipine and diltiazem on experimental atherosclerosis in cholesterol-fed rabbits. Angiology, 35: 622627. https://doi.org/10.5551/jat1973.14.3_647
[21]	Gordon, T., Castelli, W. P., Hjortland, M. C., Kannel, W. B., & Dawber, T. R. (1977). High density lipoprotein as a protective factor against coronary heart disease. The Framingham Study. The American journal of medicine, 62 (5), 707–714. https://doi.org/10.1016/0002-9343(77)90874-
[22]	Fossati, P., & Prencipe, L. (1982). Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clinical chemistry, 28 (10), 2077–2080.
[23]	Friedewald, W. T., Levy, R. I., & Fredrickson, D. S. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical chemistry, 18 (6), 499–502.
[24]	Levine, G. N., Keaney, J. F., Jr, & Vita, J. A. (1995). Cholesterol reduction in cardiovascular disease. Clinical benefits and possible mechanisms. The New England journal of medicine, 332 (8), 512–521. https://doi.org/10.1056/NEJM199502233320807
[25]	Henry, R. J. (1974). Clinical chemistry, principles and technics. 2nd Edition, Harper and Row. 525P.
[26]	Reitman S., Frankel S. Am. Journal clinicals pathology. 1957; 28: 56–60.
[27]	Yu, B. H., & Kies, C. (1993). Niacin, thiamin, and pantothenic acid bioavailability to humans from maize bran as affected by milling and particle size. Plant foods for human nutrition, 43 (1), 87-95.
[28]	Oghbaei, M., & Prakash, J. (2016). Effect of primary processing of cereals and legumes on its nutritional quality: A comprehensive review. Cogent Food & Agriculture, 2 (1), 1136015. https://doi.org/10.1080/23311932.2015.1136015
[29]	Roza, S. J., Hofstra, M. B., Van Der Ende, J., & Verhulst, F. C. (2003). Stable prediction of mood and anxiety disorders based on behavioral and emotional problems in childhood: A 14-year follow-up during childhood, adolescence, and young adulthood. American Journal of Psychiatry, 160 (12), 2116-2121. https://doi.org/10.1176/appi.ajp.160.12.2116
[30]	Zohra, BAF, Merzouk, H., Bouanane, S., Benkalfat, NB, Merzouk, SA, Mulengi, JK, & Narce, M. (2010). Evaluation of the acute toxicity of 2-hydroxy-methyl-1 (N-phthaloyltryptophyl) aziridine in Wistar rats. In Annals of Analytical Toxicology (Vol. 22, No. 3, pp. 115-121). EDP Sciences. https://doi.org/10.1051/ata/2010017
[31]	Lewis, G. F., Carpentier, A., Adeli, K., & Giacca, A. (2002). Disordered fat storage and mobilization in the pathogenesis of insulin resistance and type 2 diabetes. Endocrine reviews, 23 (2), 201-229. https://doi.org/10.1210/edrv.23.2.0461
[32]	Moore, K. L., Agur, A. M., & Dalley, A. F. (2015). Essential clinical anatomy. 736p.
[33]	Ikeda, I., Yamahira, T., Kato, M., & Ishikawa, A. (2010). Black-tea polyphenols decrease micellar solubility of cholesterol in vitro and intestinal absorption of cholesterol in rats. Journal of agricultural and food chemistry, 58 (15), 8591-8595. https://doi.org/10.1021/jf1015285
[34]	Khattab, R., Brooks, M. S. L., & Ghanem, A. (2016). Phenolic analyses of haskap berries (Lonicera caerulea L.): Spectrophotometry versus high performance liquid chromatography. International Journal of Food Properties, 19 (8), 1708-1725. https://doi.org/10.1080/10942912.2015.1084316
[35]	Laviolle, B., Annane, D., Fougerou, C., & Bellissant, E. (2012). Gluco-and mineralocorticoid biological effects of a 7-day treatment with low doses of hydrocortisone and fludrocortisone in septic shock. Intensive care medicine, 38 (8), 1306-1314.
[36]	Ngatchic, J., Douanla, N., Baudelaire, E., and Njintang, N. (2020). Lipid-lowering and anti-lipase properties of powder fractions of Dichrostachys glomerata fruits. Asian Journal of Medical Sciences. 11. 69-76. 10. 10.3126/ajms.v11i5.29229.
[37]	Shi, Y., Guo, R., Wang, X., Yuan, D., Zhang, S., Wang, J., & Wang, C. (2014). The regulation of alfalfa saponin extract on key genes involved in hepatic cholesterol metabolism in hyperlipidemic rats. PloS one, 9 (2), e88282. https://doi.org/10.1371/journal.pone.0088282
[38]	Pratt D. S. and Kaplan M. M. (2000). Evaluation of abnormal liver-enzyme results in asymptomatic patients. New England Journal of Medicine. 342: 1266-1271. 10.1056/NEJM200004273421707.
[39]	Dufour, D. R., Lott, J. A., Nolte, F. S., Gretch, D. R., Koff, R. S., & Seeff, L. B. (2000). Diagnosis and monitoring of hepatic injury. I. Performance characteristics of laboratory tests. Clinical chemistry, 46 (12), 2027-2049. https://doi.org/10.1093/clinchem/46.12.2027
[40]	Nurkhasanah, M. R. R. R., & Rahardhian, M. R. R. (2015). Hepatoprotective Effect of Hibiscus sabdariffa L Extract on 7, 12-dimethylbenz (α) antracene (DMBA) Induced Rat. Int J Biol Med Res, 6 (1), 4705-4708.
[41]	Jodynis-Liebert, J., Nowicki, M., Murias, M., Adamska, T., Ewertowska, M., Kujawska, M., & Pernak, J. (2010). Cytotoxicity, acute and subchronic toxicity of ionic liquid, didecyldimethylammonium saccharinate, in rats. Regulatory Toxicology and Pharmacology, 57 (2-3), 266-273. https://doi.org/10.1016/j.yrtph.2010.03.006

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Linda Stella Mbassi, Elie Baudelaire Djantou, Richard Marcel Nguimbou, Amadou Dicko, Nicolas Yanou Njintang. (2023). Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing. American Journal of Life Sciences, 11(2), 24-35. https://doi.org/10.11648/j.ajls.20231102.13

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Linda Stella Mbassi; Elie Baudelaire Djantou; Richard Marcel Nguimbou; Amadou Dicko; Nicolas Yanou Njintang. Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing. Am. J. Life Sci. 2023, 11(2), 24-35. doi: 10.11648/j.ajls.20231102.13

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AMA Style

Linda Stella Mbassi, Elie Baudelaire Djantou, Richard Marcel Nguimbou, Amadou Dicko, Nicolas Yanou Njintang. Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing. Am J Life Sci. 2023;11(2):24-35. doi: 10.11648/j.ajls.20231102.13

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@article{10.11648/j.ajls.20231102.13,
  author = {Linda Stella Mbassi and Elie Baudelaire Djantou and Richard Marcel Nguimbou and Amadou Dicko and Nicolas Yanou Njintang},
  title = {Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing},
  journal = {American Journal of Life Sciences},
  volume = {11},
  number = {2},
  pages = {24-35},
  doi = {10.11648/j.ajls.20231102.13},
  url = {https://doi.org/10.11648/j.ajls.20231102.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20231102.13},
  abstract = {The aim of this study was to evaluate the effect of controlled differential sieving processing (CDSp) on the content of phenolic compounds and antihyperlipidemic activity of H. sabdariffa calyxes powder. For this, four granulometric classes (H. sabdariffa calyxes powder. The granulometric class 212-315 μm showed the best concentration, higher than those of the other granulometric class and crude powder. This fraction also showed content of phenolic compounds higher than those of ethanolic extract except for p-coumaric acid, and protocatechic acid which were concentrated equally in both samples. Antihyperlipidemic activity were significantly influenced (P <0.05) by particle sizes. The best activity was obtained in the particle size classes of 212-315 µm and <180 µm which were significantly (P <0.05) higher than those of the other fractions and of the unsieved powder to improve the lipid parameters (total cholesterol, LDL-C, HDL-C and triglycerides), transaminases (ALAT and ASAT) and creatine. However, compared to the ethanolic extract, only the granulometric class of 212-315 µm showed a significantly higher activity. CDSp can be an alternative to ethanol extraction and may be an option to improve the content of phenolic compounds and antihyperlipidemic activity of plant powders.},
 year = {2023}
}

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TY  - JOUR
T1  - Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing
AU  - Linda Stella Mbassi
AU  - Elie Baudelaire Djantou
AU  - Richard Marcel Nguimbou
AU  - Amadou Dicko
AU  - Nicolas Yanou Njintang
Y1  - 2023/06/09
PY  - 2023
N1  - https://doi.org/10.11648/j.ajls.20231102.13
DO  - 10.11648/j.ajls.20231102.13
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 24
EP  - 35
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20231102.13
AB  - The aim of this study was to evaluate the effect of controlled differential sieving processing (CDSp) on the content of phenolic compounds and antihyperlipidemic activity of H. sabdariffa calyxes powder. For this, four granulometric classes (H. sabdariffa calyxes powder. The granulometric class 212-315 μm showed the best concentration, higher than those of the other granulometric class and crude powder. This fraction also showed content of phenolic compounds higher than those of ethanolic extract except for p-coumaric acid, and protocatechic acid which were concentrated equally in both samples. Antihyperlipidemic activity were significantly influenced (P <0.05) by particle sizes. The best activity was obtained in the particle size classes of 212-315 µm and <180 µm which were significantly (P <0.05) higher than those of the other fractions and of the unsieved powder to improve the lipid parameters (total cholesterol, LDL-C, HDL-C and triglycerides), transaminases (ALAT and ASAT) and creatine. However, compared to the ethanolic extract, only the granulometric class of 212-315 µm showed a significantly higher activity. CDSp can be an alternative to ethanol extraction and may be an option to improve the content of phenolic compounds and antihyperlipidemic activity of plant powders.
VL  - 11
IS  - 2
ER  -

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Author Information

Linda Stella Mbassi

Institute of Agricultural Research for Development (IRAD), Ngaoundere, Cameroon
Elie Baudelaire Djantou

Department of Food Sciences and Nutrition, National School of Agro-industrial Science, University of Ngaoundere, Ngaoundere, Cameroon
Richard Marcel Nguimbou

Department of Food Sciences and Nutrition, National School of Agro-industrial Science, University of Ngaoundere, Ngaoundere, Cameroon
Amadou Dicko

University of Lorraine, Chemistry and Physics Laboratory - Multi-Scale Approach to Complex Media, Metz, France
Nicolas Yanou Njintang

Department of Biological Sciences, Faculty of Sciences, University of Ngaoundere, Ngaoundere, Cameroon

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Linda Stella Mbassi, Elie Baudelaire Djantou, Richard Marcel Nguimbou, Amadou Dicko, Nicolas Yanou Njintang. (2023). Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing. American Journal of Life Sciences, 11(2), 24-35. https://doi.org/10.11648/j.ajls.20231102.13

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ACS Style

Linda Stella Mbassi; Elie Baudelaire Djantou; Richard Marcel Nguimbou; Amadou Dicko; Nicolas Yanou Njintang. Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing. Am. J. Life Sci. 2023, 11(2), 24-35. doi: 10.11648/j.ajls.20231102.13

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AMA Style

Linda Stella Mbassi, Elie Baudelaire Djantou, Richard Marcel Nguimbou, Amadou Dicko, Nicolas Yanou Njintang. Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing. Am J Life Sci. 2023;11(2):24-35. doi: 10.11648/j.ajls.20231102.13

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@article{10.11648/j.ajls.20231102.13,
  author = {Linda Stella Mbassi and Elie Baudelaire Djantou and Richard Marcel Nguimbou and Amadou Dicko and Nicolas Yanou Njintang},
  title = {Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing},
  journal = {American Journal of Life Sciences},
  volume = {11},
  number = {2},
  pages = {24-35},
  doi = {10.11648/j.ajls.20231102.13},
  url = {https://doi.org/10.11648/j.ajls.20231102.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20231102.13},
  abstract = {The aim of this study was to evaluate the effect of controlled differential sieving processing (CDSp) on the content of phenolic compounds and antihyperlipidemic activity of H. sabdariffa calyxes powder. For this, four granulometric classes (H. sabdariffa calyxes powder. The granulometric class 212-315 μm showed the best concentration, higher than those of the other granulometric class and crude powder. This fraction also showed content of phenolic compounds higher than those of ethanolic extract except for p-coumaric acid, and protocatechic acid which were concentrated equally in both samples. Antihyperlipidemic activity were significantly influenced (P <0.05) by particle sizes. The best activity was obtained in the particle size classes of 212-315 µm and <180 µm which were significantly (P <0.05) higher than those of the other fractions and of the unsieved powder to improve the lipid parameters (total cholesterol, LDL-C, HDL-C and triglycerides), transaminases (ALAT and ASAT) and creatine. However, compared to the ethanolic extract, only the granulometric class of 212-315 µm showed a significantly higher activity. CDSp can be an alternative to ethanol extraction and may be an option to improve the content of phenolic compounds and antihyperlipidemic activity of plant powders.},
 year = {2023}
}

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TY  - JOUR
T1  - Improvement of Phenolic Compounds and Antihyperlipidemic Activity of Hibiscus sabdariffa L. Calyxes Powder Using CDS Processing
AU  - Linda Stella Mbassi
AU  - Elie Baudelaire Djantou
AU  - Richard Marcel Nguimbou
AU  - Amadou Dicko
AU  - Nicolas Yanou Njintang
Y1  - 2023/06/09
PY  - 2023
N1  - https://doi.org/10.11648/j.ajls.20231102.13
DO  - 10.11648/j.ajls.20231102.13
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 24
EP  - 35
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20231102.13
AB  - The aim of this study was to evaluate the effect of controlled differential sieving processing (CDSp) on the content of phenolic compounds and antihyperlipidemic activity of H. sabdariffa calyxes powder. For this, four granulometric classes (H. sabdariffa calyxes powder. The granulometric class 212-315 μm showed the best concentration, higher than those of the other granulometric class and crude powder. This fraction also showed content of phenolic compounds higher than those of ethanolic extract except for p-coumaric acid, and protocatechic acid which were concentrated equally in both samples. Antihyperlipidemic activity were significantly influenced (P <0.05) by particle sizes. The best activity was obtained in the particle size classes of 212-315 µm and <180 µm which were significantly (P <0.05) higher than those of the other fractions and of the unsieved powder to improve the lipid parameters (total cholesterol, LDL-C, HDL-C and triglycerides), transaminases (ALAT and ASAT) and creatine. However, compared to the ethanolic extract, only the granulometric class of 212-315 µm showed a significantly higher activity. CDSp can be an alternative to ethanol extraction and may be an option to improve the content of phenolic compounds and antihyperlipidemic activity of plant powders.
VL  - 11
IS  - 2
ER  -

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